Effect of the different dimeric forms of the platelet‐derived growth factor on cellular responses in mouse Swiss 3T3 fibroblasts
- 11 September 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 255 (1) , 191-195
- https://doi.org/10.1016/0014-5793(89)81089-0
Abstract
PDGF consists of two polypeptide chains, A and B, and all three possible dimers have been isolated from different sources. Human PDGF, essentially AB, porcine PDGF (BB) and recombinant PDGF-AA were tested on Swiss 3T3 fibroblasts for their ability to stimulate mitogenesis, phosphoinositide turnover and tyrosine phosphorylation of the PDGF receptor. When used in saturating amounts, the three isoforms were equally active in inducing mitogenesis. However, PDGF-AA was less active than AB and BB to induce the phosphorylation of the receptor and the turnover of phosphoinositides (30% and 50%, respectively). These findings suggest that, in Swiss 3T3 fibroblasts, PDGF receptors of the α-type are present in a slightly lower amount than β-type. In addition, the two types of receptor appear to have similar physiological functions.Keywords
This publication has 29 references indexed in Scilit:
- Isolation of a Novel Receptor cDNA Establishes the Existence of Two PDGF Receptor GenesScience, 1989
- Dissociation of the ligand and dephosphorylation of the platelet‐derived growth factor receptorFEBS Letters, 1988
- A Common PDGF rEceptor Is Activated by Homodimeric A and B Forms of PDGFScience, 1988
- Two Classes of PDGF Receptor Eecognize Different Isoforms of PDGFScience, 1988
- A glioma-derived PDGF a chain homodimer has different functional activities from a PDGF AB heterodimer purified from human plateletsCell, 1988
- Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptorsNature, 1986
- PDGF-induced receptor phosphorylation and phosphoinositide hydrolysis are unaffected by protein kinase C activation in mouse Swiss 3T3 and human skin fibroblastsBiochemical and Biophysical Research Communications, 1986
- cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell linesNature, 1986
- In vivo phosphorylation and dephosphorylation of the platelet-derived growth factor receptor studied by immunoblot analysis with phosphotyrosine antibodiesBiochimica et Biophysica Acta (BBA) - General Subjects, 1986
- A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chainsNature, 1986