Freeze-Drying Injury of Lactobacillus Acidophilus

Abstract
Lactobacillus acidophilus ATCC 4355 cells were more stable to freezing at −20°C after supplementation of the growth medium with 0.1% CaCO3. However, added calcium did not affect viability and injury during lyophilization. Inclusion of 5% glycerol in the suspending medium before freeze-drying resulted in superior survival and decreased injury during the drying process.

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