Properties of platelet concentrates prepared after extended whole blood holding time

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Abstract
Extension of the maximum holding time for whole blood collected into a CPD-ADSOL system for 6 to 8 hours at ambient temperature under conditions that cause the temperature of the blood to decrease to 20 to 24.degree.C would facilitate the preparation of platelet concentrates (PCs). In this study, the properties of CPD-PCs prepared and stored for 5 days in PL-732 containers after various initial holding periods were assessed in two laboratories, designated Laboratory A and Laboratory B. Laboratory A found higher platelet-rich plasma (PRP) volumes (276 .+-. 25 vs. 249 .+-. 19 mL) and platelet yields (76 .+-. 18 vs. 66 .+-. 18 .times. 109 platelets) with 8-hour holds (n = 10) than with 1- to 2-hour holds (n = 10), although only the differences in PRP volumes was significant (p < 0.05). No significant difference was observed in autologous in vivo recovery (54 .+-. 11 vs. 47 .+-. 9%) or survival (167 .+-. 37 vs. 170 .+-. 25 hrs), as calculated by gamma function using 111In as radiolabel. Laboratory B also found higher PRP volumes (304 .+-. 31 vs. 279 .+-. 37 mL) and platelet yields with 8-hour holds (n = 12) than with a 6-hour holds (n = 10) (88 .+-. 26 vs. 77 .+-. 27 .times. 109 platelets). No significant differences were found in morphology score, the extent of release of .beta.-thromboglobulin, the discharge of lactate dehydrogenase, or hypotonic shock response. These studies showed that an 8-hour preprocessing hold period did not significantly affect in vitro and in vivo properties of PCs after 5 days of storage.

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