Poly a blocks in the nuclear ribonucleoprotein complexes, containing pre-mRNA
- 1 December 1973
- journal article
- research article
- Published by Springer Nature in Molecular Biology Reports
- Vol. 1 (4) , 193-199
- https://doi.org/10.1007/bf00357641
Abstract
Poly A was found in nuclear particles containing pre-mRNA. It was shown that during the isolation of 30S particles from rat liver or Ehrlich ascites carcinoma nuclei, all poly A is detached from the particles containing pre-mRNA and is found in the form of RNP with a sedimentation coefficient of about 14S. When RNP polyparticles are isolated in the presence of RNase inhibitor poly A is distributed among the particles of higher molecular weights. Since the sedimentation properties and buoyant density of the poly A-containing particles are different from the 30S particles it was suggested that the poly A fragments are bound not with informofers, but with another kind of protein.Keywords
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