Apisimin, a new serine–valine‐rich peptide from honeybee (Apis mellifera L.) royal jelly: purification and molecular characterization1
Open Access
- 28 August 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 528 (1-3) , 125-129
- https://doi.org/10.1016/s0014-5793(02)03272-6
Abstract
A peptide named apisimin was found in honeybee (Apis mellifera L.) royal jelly (RJ). N‐terminal sequencing showed that this peptide corresponded to the sequence of a cDNA clone isolated from an expression cDNA library prepared from heads of nurse honeybees. No homology was found between the protein sequence of apisimin with a molecular mass of 5540.4 Da and sequences deposited in the Swiss‐Prot database. The 54 amino acids of apisimin do not include Cys, Met, Pro, Arg, His, Tyr, and Trp residues. The peptide shows a well‐defined secondary structure as observed by CD spectroscopy, and has the tendency to form oligomers. Isoelectrofocusing showed apisimin to be an acidic peptide.Keywords
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