Protein staining of 6 mm diameter sodium dodecyl sulfate‐polyacrylamide gels within one and a half hours
- 1 January 1985
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 6 (8) , 408-409
- https://doi.org/10.1002/elps.1150060812
Abstract
A device for rapid rotation of perforated gel holding tubes has been constructed by which the diffusion of sodium dodecyl sulfate (SDS) from cylindrical polyacrylamide gels can be substantially accelerated. This device allows one to stain protein bands obtained in SDS‐polyacrylamide gel electrophoresis (9 % gel concentration) within one and a half hours, using the procedure of fixation in trichloroacetic acid followed by Coomassie Brilliant Blue G‐250 staining, which requires no destaining.Keywords
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