High-Performance Liquid Chromatographic Determination of CGA-184699 (Lufenuron) in Dog and Cat Blood

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Abstract
A method for quantitation of lufenuron (CGA-184699) in blood was developed for the purpose of determining lufenuron pharmacokinetics in cats and dogs. Lufenuron is an investigational insect development inhibitor used in the control of fleas on cats and dogs. An extract of lufenuron from blood is passed through a solid-phase extraction column before injection onto a C-18 column and elution with a mobile phase of acetonitrile and tetrahydrofuran in water. The eluate is monitored with a UV detector at 254 nm. Extraction efficiencies of lufenuron and the internal standard (CGA-112913) were 96% and 94%, respectively. The minimum detectable amount was 0.1 ng; day-to-day variation showed a CV% of less than 5%. Application of the method to a pharmacokinetic study in which one cat and dog received a single intravenous 10 mg/kg dose showed half-lives of approximately 60 days in both species.

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