THE INVESTIGATION OF THE TRANSMISSION OF BACTERIOPHAGE-T4 AMBER MUTANTS .2. THE TEMPERATURE SENSITIVITY OF MULTIPLICATION OF GENE-26 AMBER MUTANTS IN ESCHERICHIA-COLI-B CELLS AND THE LACK OF SUCH SENSITIVITY IN THE CASE OF GENE-33

  • 1 January 1983
    • journal article
    • research article
    • Vol. 19  (7) , 1075-1080
Abstract
The dependence of multiplication of genes 26 and 33 amber mutants of T4D phage in a non-permissive host on the temperature in the range of 25.degree.-41.degree. C has been studied. The secondary mutations observed in the genome of gene 26 mutants amS105 and amNG114 were excluded by crossing these mutants with the wild type phage. Multiplication of gene 26 amber mutants amS105x5, amN131 and amNG114x1 depends on the temperature: the burst size of phage gradually decreased by .apprx. 3 orders when the incubation temperature was changed from 25.degree. to 41.degree. C. In the case of gene 33 amber mutants, their multiplication was only slightly dependent on the temperature, maximum burst size having been observed at 37.degree. C. The decrease in the burst size at 41.degree. C of gene 33 mutant amc18 was shown to be caused by secondary ts mutation in the phage genome. It is concluded that the phenomenon of the temperature sensitivity of multiplication of T4D phage amber mutants is gene specific.