Purification of Patient-Related Allergens by Means of Bioaffinity Chromatography on a Sepharose Anti-IgE Patient IgE Immunosorbent

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Abstract
A method is described for bioaffinity chromatographic purification of patient-specific IgE reactive allergens by using sepharose coupled rabbit anti-human IgE antibodies, patient serum and an allergen extract. The conditions for this bioaffinity chromatographic procedure were optimized; the nonspecific binding of serum proteins and antigens can be kept at very low levels by a high ionic strength. The specificity of the method was determined by showing that allergens to which the patient did not react were not bound to the affinity matrix. The isolated allergens could probably be used for more specific immunotherapy in the future.