A HIGHLY SENSITIVE ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF PANCREATIC LIPASE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 20  (7) , 515-519
Abstract
A highly sensitive and specific enzyme immunoassay for the determination of pancreatic lipase is described. The test follows the sandwich principle; total incubation time is 4.5 h at 20-25.degree. C. Lipase concentrations between 3-300 .mu.g/l can be quantified (sample dilution 1 + 10); the lower detection limit is 0.3 .mu.g/l (sample dilution 1 + 1). Coefficients of variation from 2.9 to 6.5% for the in batch precision and from 4.4 to 10.5% for the day-to day precision were found. A reference range from 7.7 to 56 .mu.g/l was calculated from the lipase concentrations in 369 serum samples from healthy adults (2.5th-97.5th percentile, median 23 .mu.g/l). Very low concentrations were found in cord blood (median 2.8 .mu.g/l). The lipase level remains quite constant between the ages of 3-50 yr (median 15-20 .mu.g/l); at the age of 70 yr a median of 26 .mu.g/l is reached.