Abstract
A bacteria-free culture of the important centric diatom Skeletonema costatum has recently been established at Millport, and some exploratory nutritional experiments have been carried out with it. S. costatum is a photo-autotroph whose autotrophy is, apparently, limited to cobalamin (vitamin B12).Details concerning isolation and maintenance of S. costatum differ from those already published (Droop, 1954a, 1955) only in the matter of pH control, a vital factor in the successful culture of this species whose range of tolerance is narrow (pH 7–5–8–5).

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