Genotypic analysis using a Y‐chromosome‐specific probe following bone marrow transplantation

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Abstract
To monitor successful engraftment after bone marrow transplantation, we performed Southern hybridization analysis or dot blot analysis of DNA in a set of sex‐mismatched cases using a Y‐chromosome‐specific DNA probe (pHY10). This method was extremely sensitive and rapid for checking which cells contain the Y‐chromosome. Using this probe, analysis of cells from peripheral blood and bone marrow after transplantation demonstrated the usefulness of confirming engraftment of donor cells and of detecting mixed lymphohematopoietic chimerism.