ISOLATION OF SUBCELLULAR GRANULES FROM 2ND-STAGE JUVENILES OF MELOIDOGYNE-INCOGNITA
- 1 January 1985
- journal article
- research article
- Vol. 17 (4) , 482-488
Abstract
Subcellular granules from the second-stage (preparasitic) juveniles of root-knot nematode Meloidogyne incognita were isolated by isopycnic centrifugation on Percoll. The granules had an apparent density of 1.13 g/cm3. The relative specific activity of acid phosphatase in the granule extract was 8.4. Acid phosphatase activity was also detected histochemically in the subventral gland granules. Electron microscopy and malate dehydrogenase activity indicated that contamination of granules by mitochondrial was negligible. Electrophoresis of the granule extract in the presence of sodium dodecyl sulfate showed 15-20 major proteins bands.Keywords
This publication has 7 references indexed in Scilit:
- Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivityAnalytical Biochemistry, 1981
- Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for proteinAnalytical Biochemistry, 1981
- TECHNIQUE FOR COLLECTION OF LARVAE OF MELOIDOGYNE SPP AND A COMPARISON OF EGGS AND LARVAE AS INOCULA1977
- [19] Intra- and extramitochondrial malate dehydrogenases from chicken and tuna heartPublished by Elsevier ,1969
- Changes Associated with Parasitism in Nematodes. II. Histochemical and Microspectrophotometric Analyses of Preparasitic and Parasitic Larvae of Meloidogyne javanicaJournal of Parasitology, 1967
- [10] Assay of inorganic phosphate, total phosphate and phosphatasesPublished by Elsevier ,1966
- Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liverBiochemical Journal, 1955