CONTINUOUS HUMAN-BONE MARROW CULTURE - IA-ANTIGEN CHARACTERIZATION OF PROBABLE PLURIPOTENTIAL STEM-CELLS

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 55  (4) , 682-690
Abstract
The presence of Ia[immune response-associated]-like antigens on human CFU-c [granulocytic progenitor cells] and BFUe [erythropoietic burst-forming units] was confirmed and a cell type that lacked immediate capacity for granulocytic colony formation but generated CFU-c after brief incubation in simple suspension culture was identified. This pre-CFU-c and its immediate progeny were extremely sensitive to killing by anti-Ia serum with complement. Anti-Ia serum plus complement treatment of human bone marrow, while eliminating 93-97% of all CFU-c and BFU-e, did not prevent rapid regeneration of these progenitor cells and their production for some weeks under the conditions of continuous marrow culture. The human equivalent of the pluripotential stem cell can apparently replicate for some weeks in culture and generate committed progenitors such as CFU-c and BFU-e. Ia-like antigen is apparently absent on the pluripotential stem cell, is apparently rapidly gained as commitment to the various progenitor cell types occur and is apparently subsequently lost as these latter undergo differentiation within the marrow.