THE FRACTIONATION OF PHOSPHORUS CONTAINING CONSTITUENTS IN THE ALLANTOIC MEMBRANE OF THE EMBRYONATED EGG

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Abstract
A procedure is described which permitted the phosphorus containing constituents in allantoic membranes of embryonated eggs to be separated into fractions as follows: alcohol soluble phosphorus from which the phospholipids were separated, phosphorus soluble in 5% trichloroacetic acid in which inorganic phosphorus was determined, and nucleic acid phosphorus which was further separated into pentose and desoxypentose nucleic acids. This procedure was applied to membranes between 9 and 13 days of age. It was found that the total phosphorus amounted to 9 to 10 mgm. per gm. of dried tissue. Alcohol soluble phosphorus accounted for approximately 28%, acid soluble phosphorus for 37%, and nucleic acid phosphorus for 35% of the total phosphorus. About half the acid soluble phosphorus was inorganic and about 85% of the alcohol soluble phosphorus was associated with phospholipids. These proportions remained essentially constant over the period studied. The ratio of pentose to desoxypentose nucleic acid phosphorus also remained fairly constant over the interval at about 2.2. There was no significant difference in the amounts of P32 taken up over a period of 72 hr. by normal allantoic membranes and those infected with influenza virus, when inorganic radioactive phosphorus was placed in the allantoic sacs of 11-day embryonated eggs.