Abstract
Experiments were done in an attempt to ascertain the significance of the mandibular glands of nurse bees in female caste differentiation. Groups of 30 or 75, 10-day-old nurse bees, with their mandibular glands removed, were caged in the laboratory. Each group was provided with female larvae in plastic queen cell cups. The treated nurse bees were able to rear a few pupae, from these larvae, which were classified as queenlike intermediates while the untreated nurse bees reared pupae or adults which were classified as queens or queenlike intermediates.

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