COMPARATIVE STUDIES OF GLUCOSE-METABOLISM IN HTC, RLC, MH1C1, AND REUBER H35 RAT HEPATOMA-CELLS
- 1 January 1979
- journal article
- research article
- Vol. 39 (3) , 1051-1055
Abstract
Carbohydrate metabolism by 4 rat hepatoma cell lines in culture, namely, Reuber H35, MH1C1, RLC, and HTC, was investigated. Glucose utilization by H35 and MH1C1 cells is lower than that by RLC and HTC cells. The 4 cell lines differ with respect to the accumulation of lactic acid in the growth medium; in particular, H35 cells show uptake of lactic acid, rather than accumulation in the medium. Specific activities of a number of enzymes involved in glycolysis, gluconeogenesis, pentose phosphate pathway and glycogen formation were determined in the 4 cell lines. A direct relationship between the differences was found for the activities of some enzymes belonging to carbohydrate metabolism, namely hexokinase, pyruvate kinase, aldolases A and B, G-6-P dehydrogenase and phosphogluconate dehydrogenase. Differences in glucose utilization by the different cell lines were found.This publication has 8 references indexed in Scilit:
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