Properties of 2′,3′‐dideoxy‐2′,3′‐dehydrothymidine 5′‐triphosphate in terminating DNA synthesis catalyzed by several different DNA polymerases
- 13 July 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 219 (1) , 151-155
- https://doi.org/10.1016/0014-5793(87)81208-5
Abstract
2′‐3′‐Dideoxy‐2′,3′‐dehydrothymidine 5′‐triphosphate (dddTTP) shows termination substrate properties in the DNA synthesis catalyzed by E. coli DNA polymerase I KF, rat liver DNA polymerase β, reverse transcriptases of avian myeloblastosis virus and Raus sarcoma virus and calf thymus terminal deoxynucleotidyl transferase. This implies that the mononucleotide residue of dddTTP incorporates into 3′‐termini of newly synthesized DNA chains. However, dddTTP has no influence on the DNA synthesis catalyzed by calf thymus DNA polymerase α. In the case of some DNA polymerases dddTTP was one order of magnitude more effective in comparison with the other known termination substrates.Keywords
This publication has 9 references indexed in Scilit:
- Nucleoside 5′-triphosphates with modified sugars as substrates for DNA polymerasesBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1986
- Nucleoside 5′-triphosphates modified at sugar residues as substrates for calf thymus terminal deoxynucleotidyl transferase and for AMV reverse transcriptaseBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1986
- 3′-Hydroxymethyl 2′-deoxynucleoside 5′-triphosphates are inhibitors highly specific for reverse transcriptaseFEBS Letters, 1986
- NMR studies of the conformations and interactions of substrates and ribonucleotide templates bound to the large fragment of DNA polymerase IBiochemistry, 1986
- Nuclear Overhauser effect studies of the conformations and binding site environments of deoxynucleoside triphosphate substrates bound to DNA polymerase I and its large fragmentBiochemistry, 1985
- 3'-Fluoro-2',3'-dideoxyribonucleoside 5'-triphosphates: terminators of DNA synthesisFEBS Letters, 1985
- Purification of a 9S DNA polymerase .alpha. species from calf thymusBiochemistry, 1981
- Enzymic synthesis of deoxyribonucleic acid. XXXIV. Termination of chain growth by a 2',3'-dideoxyribonucleotideBiochemistry, 1969
- Nucleosides. IX. The Formation of 2',3'-Unsaturated Pyrimidine Nucleosides via a Novel β-Elimination Reaction1,2The Journal of Organic Chemistry, 1966