Acetylcholine Receptor: Covalent Attachment of Depolarizing Groups at the Active Site
- 20 June 1969
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 164 (3886) , 1420-1421
- https://doi.org/10.1126/science.164.3886.1420
Abstract
Following reduction of the acetylcholine receptor in the electroplax with dithiothreitol, the quaternary ammonium compounds bromoacetylcholine bromide and the p -nitrophenyl ester of ( p -carboxyphenyl) trimethylammonium iodide react near the active site probably with a sulfhydryl group. The covalently attached quaternary ammonium moieties additionally interact with the active site noncovalently to activate the receptor and cause depolarization of the cell.Keywords
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