CHARACTERIZATION OF HUMAN ANTI-FACTOR-8 ANTIBODIES PURIFIED BY IMMUNE-COMPLEX FORMATION

  • 1 January 1976
    • journal article
    • research article
    • Vol. 48  (6) , 931-939
Abstract
Anti-facor VIII antibodies from 3 hemophilia A patients were selectively isolated by immune complex formation and secondary precipitation with polyethylene glycol. These antibodies were characterized as IgG [immunoglobulin G], with only 1 type of L chains (.kappa.) and a mixture of IgG3 and IgG4 H chains. This finding indicated that stable complexes of factor VIII and homologous anti-factor VIII antibodies could be obtained, and that the immunologic response to the infusion of homologous factor VIII was oligoclonal rather than monoclonal in these patients.