A Generalized Theoretical Treatment of the Transient-state Kinetics of Enzymic Reaction Systems Far from Equilibrium.

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Abstract
The transient-state kinetics of enzyme systems far from equilibrium were analyzed by a theoretical treatment of the generalized King-Altman mechanism for enzyme reactions. Conditions are defined under which the kinetic differential equations for this generalized mechanism can be analytically solved. The structure of the analytical solution is characterized. The practical implications of the theoretical results are discussed with reference to the planning, evaluation and interpretation of transient-state kinetic experiments performed by stopped-flow techniques.