INFLUENCE OF TIME IN CULTURE ON CELLULAR AND NEURONAL PROPERTIES OF NEUROBLASTOMA X GLIOMA HYBRID-CELLS - WITH AN APPENDIX - MATHEMATICAL-DESCRIPTION OF KINETICS OF LOSS IN CELL-VOLUME
- 1 January 1977
- journal article
- research article
- Vol. 27 (OCT) , 141-155
Abstract
The stability of a clonal mouse neuroblastoma .times. rat glioma hybrid cell line was examined. Cell volume and cellular content of DNA and protein decreased with the number of serial subcultivations. Measurements of cell volume could be used to monitor the loss of DNA from hybrid cells. After 60 passages a stable population of hybrid cells arose. A mathematical model for the simultaneous loss of cellular volume, DNA and protein was introduced. After 60 to 70 subcultivations, the hybrid cells were still capable of extending processes, action potentials could still be elicited, the cells still responded to prostaglandin E1 and choline acetyltransferase specific activity decreased substantially.This publication has 2 references indexed in Scilit:
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- STUDIES OF INTERSPECIFIC (RAT x MOUSE) SOMATIC HYBRIDS. I. ISOLATION, GROWTH AND EVOLUTION OF THE KARYOTYPEGenetics, 1966