Changes in the concentration of cyclic nucleotides dependent on their antibodies in plasma.

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Abstract
In the process of immunization of rabbits with cAMP or cGMP, the plasma concentration of cAMP or cGMP was markedly elevated as the titer of the antiserum increased. The i.v. injection of anti-cAMP or anti-cGMP antiserum into rats or mice also caused a marked increase in the respective cyclic nucleotide in plasma. An injection with the antiserum did not cause any significant increase in urinary cyclic nucleotides. The effect of the antiserum seems to be different from that of glucagon or carbamylcholine which increases cAMP or cGMP, respectively, in plasma and urine. The injection of the antiserum, but not nephrectomy, caused a significant increase in plasma cyclic nucleotides in adrenalectomized rats. This made it unlikely that the antiserum-induced increase was due to the inhibition of urinary excretion. The plasma cyclic nucleotides, increased by an i.v. injection with the antiserum, survived enzymatic hydrolysis by phosphodiesterase in vitro; most of them were bound to the antibody. The increases in plasma cyclic nucleotides in the process of cyclic nucleotide-immunization apparently were mainly due to decreased metabolic clearance of plasma cyclic nucleotides.