TEMPORAL CHANGES IN THE HYPOTHALAMIC AND SERUM LUTEINIZING HORMONE-RELEASING HORMONE (LH-RH) LEVELS AND THE CIRCULATING OVARIAN STEROIDS DURING THE RAT OESTROUS CYCLE

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Abstract
Cycling female rats were sacrificed at various times during the 4-day estrous cycle. LH-RH in the medial basal hypothalamus (MBH) and serum LH-RH, LH, estradiol (Oe2), and progesterone were analyzed by radioimmunoassays. The MBH LH-RH content was lowest at 19.55-20.15 h during proestrus but increased gradually through estrus and diestrus I to significantly higher values at noon of diestrus II, and then decreased precipitously at 18.00 h. Although serum LH levels remained basal from midnight of proestrus through diestrus II, serum LH-RH levels were significantly elevated at 12.00 and 21.00 h on estrus (vs. the midnight proestrus levels) and declined between 15.00 and 22.00 h of diestrus I. In conjunction with high Oe2 titers, LH-RH in the MBH and serum declined initially during proestrus between 14.07-14.45 h and then increased abruptly between 14.55-15.55 h onwards. LH-RH activity in the MBH and serum receded gradually to the early afternoon levels by 19.55-24.00 h while the peak in serum LH was observed at 17.05-18.05 h. The hypersecretion of the MBH LH-RH (synthesis + release) may be responsible for the pre-ovulatory discharge of LH.