A Modified Silver Method for Demonstrating Developing Nervous Tissue in Culture

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Abstract
Dissociated explants of 8-day-old embryonic chick cerebrum were cultured for up to 18 days. By the beginning of the 2nd week and thereafter, primary cultures of neurons exhibited characteristic differentiated morphology with an interconnecting neurofibrillary network that became increasingly ramified. Neurons in bipolar, tripolar or multipolar form could be demonstrated positively using a short modified silver impregnation method with potassium ferrocyanide.

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