The herpes simplex virus amplicon: analyses of cis-acting replication functions.
- 1 February 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (3) , 694-698
- https://doi.org/10.1073/pnas.82.3.694
Abstract
Previous studies have shown that defective virus vectors (amplicons) derived from herpes simplex viruses (HSV) could be efficiently propagated in virus stocks in the presence of trans-acting helper virus functions. The present study established that 2 separate cis-acting functions, a DNA replication origin and a cleavage/packaging signal, are required for amplicon propagation. Using delected derivatives of cloned amplicons, one of the viral DNA replication origins (ori-2 or oriL) were mapped at coordinate 0.422 of the standard HSV-1 genome and at an equivalent position within the HSV-2 genome.This publication has 13 references indexed in Scilit:
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