Copper complexes of (−)‐epicatechin gallate and (−)‐epigallocatechin gallate act as inhibitors of Ribonuclease A
- 25 July 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (19) , 4703-4708
- https://doi.org/10.1016/j.febslet.2006.07.054
Abstract
Green tea polyphenols, which have the ability to inhibit angiogenesis, form complexes with Cu(II), a known potent stimulator of blood vessel proliferation. Copper complexes of (−)‐epicatechin gallate and (−)‐epigallocatechin gallate were found to inhibit the enzymatic activity of Ribonuclease A (RNase A) as revealed by an agarose gel based assay and urea denatured gel electrophoresis. The copper complexes were found to be non‐competitive inhibitors of RNase A with inhibition constants in the micromolar range. Changes in the secondary structure of the protein are found to occur due to the interaction as revealed from Fourier transform infrared and circular dichroism studies.Keywords
This publication has 29 references indexed in Scilit:
- Interaction of (−)‐epigallocatechin‐3‐gallate with human serum albumin: Fluorescence, fourier transform infrared, circular dichroism, and docking studiesProteins-Structure Function and Bioinformatics, 2006
- Kinetics of the Inhibition of Bovine Liver Dihydrofolate Reductase by Tea Catechins: Origin of Slow-Binding Inhibition and pH StudiesBiochemistry, 2005
- Green tea polyphenols as inhibitors of ribonuclease ABiochemical and Biophysical Research Communications, 2004
- Evaluation of Epigallocatechin Gallate and Related Plant Polyphenols as Inhibitors of the FabG and FabI Reductases of Bacterial Type II Fatty-acid SynthaseJournal of Biological Chemistry, 2004
- 3′‐Azido‐3′‐deoxythymidine binding to ribonuclease A: Model for drug‐protein interactionBiopolymers, 2003
- Ribonuclease AChemical Reviews, 1998
- Inhibition of carcinogenesis by tea: The evidence from experimental studiesCritical Reviews in Food Science and Nutrition, 1997
- Protein secondary structures in water from second-derivative amide I infrared spectraBiochemistry, 1990
- Examination of the secondary structure of proteins by deconvolved FTIR spectraBiopolymers, 1986
- Some spectrophotometric and polarimetric experiments with ribonucleaseBiochimica et Biophysica Acta, 1957