A protein kinase from wheat germ that phosphorylates the largest subunit of RNA polymerase II.
Open Access
- 31 July 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 1 (8) , 827-836
- https://doi.org/10.1105/tpc.1.8.827
Abstract
A protein kinase from wheat germ that phosphorylates the largest subunit of RNA polymerase IIA has been partially purified and characterized. The kinase has a native molecular weight of about 200 kilodaltons. This kinase utilizes Mg2+ and ATP and transfers about 20 phosphates to the heptapeptide repeats Pro-Thr-Ser-Pro-Ser-Tyr-Ser in the carboxyl-terminal domain of the 220-kilodalton subunit of soybean RNA polymerase II. This phosphorylation results in a mobility shift of the 220-kilodalton subunits of a variety of eukaryotic RNA polymerases to polypeptides ranging in size from greater than 220 kilodaltons to 240 kilodaltons on sodium dodecyl sulfate-polyacrylamide gels. The phosphorylation is highly specific to the heptapeptide repeats since a degraded subunit polypeptide of 180 kilodaltons that lacks the heptapeptide repeats is poorly phosphorylated. Synthetic heptapeptide repeat multimers inhibit the phosphorylation of the 220-kilodalton subunit.Keywords
This publication has 36 references indexed in Scilit:
- Phosphorylation of eukaryotic DNA-dependent RNA polymerase. Identification of calf thymus RNA polymerase subunits phosphorylated by two purified protein kinases, correlation with in vivo sites of phosphorylation in HeLa cell RNA polymerase II.Journal of Biological Chemistry, 1981
- Auxin induced deoxyribonucleic acid dependent ribonucleic acid polymerase activities in mature soybean hypocotylBiochemistry, 1980
- The amounts, subunit structures, and template-engaged activities of RNA polymerases in germinating soybean axesDevelopmental Biology, 1980
- Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genesNature, 1980
- Selective and accurate initiation of transcription at the ad2 major late promotor in a soluble system dependent on purified rna polymerase ii and dnaCell, 1979
- Plant DNA-dependent RNA polymerases: subunit structures and enzymic properties of the class II enzymes from quiescent and proliferating tissuesBiochemistry, 1978
- Eukaryotic RNA polymerases: comparative subunit structures, immunological properties, and .alpha.-amanitin sensitivities of the class II enzymes from higher plantsBiochemistry, 1978
- In vitro proteolysis of a large subunit of Drosophila melanogaster RNA polymerase BFEBS Letters, 1976
- Large-scale purification of wheat germ DNA-dependent RNA polymerase IIBiochemistry, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970