A simple method for typing clinical isolates of herpesvirus simplex: replication in the presence of 2h-1,3-oxazine-2,6 (3h)-dione (oxauracil)
- 1 January 1980
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 6 (1) , 21-27
- https://doi.org/10.1002/jmv.1890060104
Abstract
A rapid simple method for the typing of wild HSV isolates based on selective inhibition of HSV‐2 replication by the pyrimidine analogue oxauracil (2H‐1,3‐oxazine‐2,6(3H)‐dione) has been developed. Chi‐square analysis of the results of typing of 30 wild strains by serum neutralization tests, immunofluorescence, and oxauracil‐inhibition indicates no significant difference in assignment of types to the isolates. The oxauracil‐inhibition test obviates confusion of typing resulting from use of immune sera containing cross‐reactive antibodies. We conclude oxauracil typing is a simple, rapid, and precise test which can be performed in any laboratory capable of isolating HSV.Keywords
This publication has 2 references indexed in Scilit:
- Intranasal Challenge of Mice with Herpes Simplex Vims: An Experimental Model for Evaluation of the Efficacy of Antiviral DrugsThe Journal of Infectious Diseases, 1976
- 2H-1,3-Oxazine-2,6(3H)-dione, a new pyrimidine antimetaboliteBiochemical and Biophysical Research Communications, 1973