Stable integration of mouse DNA into Ia-negative human B-lymphoma cells causes reexpression of the human Ia-positive phenotype.
- 1 October 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (19) , 7415-7418
- https://doi.org/10.1073/pnas.83.19.7415
Abstract
RJ 2.2.5, a variant of the human B-lymphoma cell line Raji, does not express the HLA-DR,-DQ, and -DP class II (or Ia) histocompatibility antigens, as a result of a defect in the transcription of the corresponding genes. This defect is corrected after fusion of RJ 2.2.5 cells with mouse Ia-positive cells. Previous work showed that the trans-acting transcriptional activator supplied by the mouse cells is encoded by a locus on mouse chromosome 16. We show here that reexpression of human major histocompatibility complex class II genes by RJ 2.2.5 cells can also be achieved by stable integration of mouse genomic sequences into the RJ 2.2.5 genome after DNA-mediated gene transfer.This publication has 16 references indexed in Scilit:
- aIr-1, a newly found locus on mouse chromosome 16 encoding a trans-acting activator factor for MHC class II gene expression.The Journal of Experimental Medicine, 1986
- Trans-acting element(s) operating across species barriers positively regulate expression of major histocompatibility complex class II genes.The Journal of Experimental Medicine, 1985
- Reactivation by a trans-acting factor of human major histocompatibility complex Ia gene expression in interspecies hybrids between an Ia-negative human B-cell variant and an Ia-positive mouse B-cell lymphoma.Proceedings of the National Academy of Sciences, 1985
- Structure, Sequence and Polymorphism in the HLA‐D RegionImmunological Reviews, 1985
- Gene transfer in lymphoid cells: expression of the Thy-1.2 antigen by Thy-1.1 BW5147 lymphoma cells transfected with unfractionated cellular DNA.Proceedings of the National Academy of Sciences, 1984
- Analysis of the structural heterogeneity and polymorphism of human Ia antigens. Four distinct subsets of molecules are coexpressed in the Ia pool of both DR1,1 homozygous and DR3,W6 heterozygous B cell lines.The Journal of Experimental Medicine, 1984
- Human B cell variants immunoselected against a single Ia antigen subset have lost expression of several Ia antigen subsets.The Journal of Experimental Medicine, 1983
- Detection of genes coding for human differentiation markers by their transient expression after DNA transfer.Proceedings of the National Academy of Sciences, 1982
- Human Ia molecules carrying DC1 determinants differ in both α- and β-subunits from Ia molecules carrying DR determinantsNature, 1981
- Transformation of rat cells by DNA of human adenovirus 5Virology, 1973