Antibody-mediated targeting of α-1,4-glucosidase-albumin polymers to rat hepatocytes A model for enzyme therapy

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Abstract
Chemically cross-linking .alpha.-1,4-glucosidase, homologous albumin and antibody IgG molecules raised against isolated rat hepatocytes yields an active and stable soluble enzyme-polymer complex of MW .apprx. 106. After i.v. injection, the 125I-labeled complex is preferentially associated with hepatocytes when compared with labeled free .alpha.-1,4-glucosidase, enzyme-albumin polymers without IgG or polymer linked to a non-specific IgG molecule, all of which are associated to a much larger extent with the Kupffer cells. The procedure offers several advantages for targeting of enzymes to specific tissues and cells and for the possible lowering of hepatocyte glycogen content in Type II glycogenesis (Pompe''s disease).