Genetic Analyses of Two Large-Lesion Isolates of Cucumber Mosaic Virus

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Abstract
Four strains of cucumber mosaic virus (CMV) induced small (.ltoreq. 0.1 cm diameter) necrotic lesions upon inoculation to cowpea, Vigna unguiculata ssp. cylindrica ''Catjang''. In addition, a few large (0.4-0.6 cm) necrotic lesions appear at the rate of 0.11-5.26% (0.53% average) of total lesions. These large lesion-producing isolates were mutants derived from their parent strains. Pseudorecombination analysis showed that RNA 2 carries the genetic determinant for the large-lesion phenotype in 2 large-lesion isolates derived from CMV-C and CMV-N strains. In addition, RNA 1 of a large-lesion isolate of CMV-N was responsible for induction of necrotic local lesions on cotyledons of Cucurbita pepo cv. President and the inability to move systemically in Nicotiana tabacum cv. H-423.
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