Formation of sulphmyoglobin during expression of horse heart myoglobin in Escherichia coli
- 7 March 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 340 (3) , 281-286
- https://doi.org/10.1016/0014-5793(94)80154-1
Abstract
Expression of recombinant horse heart myoglobin in Escherichia coli has been found to result in the production of both native and variable amounts (~ 16–17% total) of two sulphmyoglobin isomers. The recombinant sulphmyoglobin produced consists primarily of the A and B isomers as identified by 1H NMR spectroscopy with no evidence for production of the C isomer. Conversion of recombinant sulphmyoglobin to the native protein can be achieved by reconstitution with protohaem IX. The possible relationship of this observation to recombinant expression of other heme proteins is discussed.Keywords
This publication has 28 references indexed in Scilit:
- Analysis of antibodies and other large glycoproteins in the mass range of 150,000-200,000 daltons by electrospray ionization mass spectrometryAnalytical Chemistry, 1992
- Expression in Escherichia coli of a synthetic gene coding for horse heart myoglobinProtein Engineering, Design and Selection, 1991
- Recombinant cytochrome c peroxidase folds properly without conformational “annealing”Biochemical and Biophysical Research Communications, 1991
- Apomyoglobin as a molecular recognition surface: expression, reconstitution and crystallization of recombinant porcine myoglobin in Escherichia coliProtein Engineering, Design and Selection, 1988
- Multiple forms of sulfmyoglobin as detected by 1H nuclear magnetic resonance spectroscopyBiochemical and Biophysical Research Communications, 1986
- SulfhemoglobinemiaNew England Journal of Medicine, 1984
- Heme orientational disorder in reconstituted and native sperm whale myoglobinJournal of Molecular Biology, 1983
- Origin of the labile sulfide in the iron-sulfur proteins of EscherichiacoliBiochemical and Biophysical Research Communications, 1983
- Cleavage of the haem-protein link by acid methylethylketoneBiochimica et Biophysica Acta, 1959
- On the combinations of methæmoglobin with H 2 SProceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character, 1933