New Method for Evaluating Bronchomotor and Bronchosecretory Activities: Effects of Prostaglandins and Antigen

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Abstract
A new method was devised for measuring responses of canine airway musculature, bronchosecretion and bronchial vasculature simultaneously, in situ and the effects of prostaglandins F2.alpha. and E2 and antigen-antibody reaction by using this model were investigated. The right bronchial artery was perfused with blood at a constant flow under artificial respiration. Airway musculature response was measured as a change in ventilation overflow with a modification of the Konzett-Rossler method; the airway secretory activity was measured with the stopper method for secretion volume and with the glass plate method for viscosity. Close intraarterial injections of prostaglandin (PG) F2.alpha., 0.01-10 .mu.g, into the right bronchial artery produced bronchomuscular and vascular constrictions, while those of PGE2, 0.01-3 .mu.g, produced dilatation in a dose dependent manner. A close intraarterial injection of 1 mg protein of Ascaris suum antigen to dogs with positive skin reaction produced bronchoconstriction and vascular dilatation. Thirty min intraarterial infusions of PGF2.alpha., 0.3-3 .mu.g/min, and Ascaris antigen, 0.03-0.3 mg protein/min, resulted in a dose-dependent increase in the volume of airway secretions, while those of PGE2.alpha., 0.1-1.0 .mu.g/min, did not alter the secretory activity. Prostaglandin F2.alpha. in relatively high doses increases both bronchomotor tone and bronchosecretory activity, as does antigen-antibody reaction with Ascaris antigen. The present method is useful for evaluating effects of drugs on the respiratory tract.

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