Mosaic Qβ coats as a new presentation model
- 10 July 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 431 (1) , 7-11
- https://doi.org/10.1016/s0014-5793(98)00716-9
Abstract
The new protein carrier was developed on the basis of recombinant RNA phage Qβ capsid. C‐terminal UGA extension of the short form of Qβ coat, so‐called A1 extension, served as a target for presentation of foreign peptides on the outer surface of mosaic Qβ particles. In conditions of enhanced UGA suppression, the proportion of A1‐extended to short coats in mosaic particles dropped from 48% to 14%, with an increase of the length of A1 extension. A model insertion, short preS1 epitope 31‐DPAFR‐35 of hepatitis B surface antigen, demonstrated superficial location on the mosaic Qβ particles and ensured specific antigenicity and immunogenicity.Keywords
This publication has 20 references indexed in Scilit:
- The crystal structure of bacteriophage GA and a comparison of bacteriophages belonging to the major groups of Escherichia coli levivirusesJournal of Molecular Biology, 1997
- Phase display of proteinsCurrent Opinion in Biotechnology, 1996
- The crystal structure of bacteriophage Qβ at 3.5 å resolutionStructure, 1996
- Recombinant rna phage qβ capsid particles synthesized and self-assembled in escherichia coliGene, 1993
- Enhanced readthrough of opal (UGA) stop codons and production of Mycoplasma pneumoniae P1 epitopes in Escherichia coliGene, 1993
- Analysis of RNA phage fr coat protein assembly by insertion, deletion and substitution mutagenesisProtein Engineering, Design and Selection, 1993
- Multiple presentation of foreign peptides on the surface of an RNA-free spherical bacteriophage capsidJournal of General Virology, 1993
- Multiple display of foreign peptides on a filamentous bacteriophageJournal of Molecular Biology, 1991
- The readthrough protein A1 is essential for the formation of viable Qβ particlesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- A single UGA codon functions as a natural termination signal in the coliphage Qβ coat protein cistronJournal of Molecular Biology, 1973