Similarity of Ribulose-1,5-bisphosphate Carboxylases of Isogenic Diploid and Tetraploid Ryegrass (Lolium perenne L.) Cultivars
- 1 March 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 67 (3) , 530-534
- https://doi.org/10.1104/pp.67.3.530
Abstract
Partially purified ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) was isolated from diploid cultivars of ryegrass (L. perenne L.) using 2 separate methods. The apparent Km (CO2) values for the enzymes prepared by either method did not differ significantly between diploid and tetraploid when assayed by 2 separate techniques. The unpurified enzymes from freshly lysed (and fully functional) protoplasts of both diploid and tetraploid cultivars gave virtually identical apparent Km (CO2) values. There was no indication of large differences in affinity for CO2 of illuminated intact protoplasts from the 2 cultivars.This publication has 22 references indexed in Scilit:
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