Mass characteristics of DNA obtained from chromosomes of a human carcinoma cell line
- 1 January 1985
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 92 (3) , 234-241
- https://doi.org/10.1007/bf00348699
Abstract
Sedimentation studies of DNA from chromosomes extracted from human mitotic cells showed that highmolecular-weight DNA can be obtained if cell hypotonic treatments and prolonged metaphase blocks are avoided. Two types of large double-stranded DNA were observed. One of these (M r = 2.5×108) appeared as a size class with characteristics reminiscent of the chromosomal DNA subunit hypothesis. However, this DNA is the decay product of larger molecules, whose minimum molecular weight is 6×108.This publication has 23 references indexed in Scilit:
- DNA Double Strand Breaks in Ehrlich Ascites Tumour Cells at Low Doses of X-rays. I. Determination of Induced Breaks by Centrifugation at Reduced SpeedInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1982
- A quantitative test of Zimm's model for the rotor‐speed‐department sedimentation of linear DNA moleculesBiopolymers, 1980
- Rapid isolation of metaphase chromosomes containing high molecular weight DNA.The Journal of cell biology, 1979
- Length distributions of single-stranded DNA in Chinese hamster ovary cellsJournal of Molecular Biology, 1975
- Conformation of DNA in alkaline sucrose: The subunit hypothesis in mammalian cellsBiochemical and Biophysical Research Communications, 1974
- Anomalies in sedimentation: IV. Decrease in sedimentation coefficients of chains at high fieldsBiophysical Chemistry, 1974
- Mammalian Metaphase Chromosomes with High Molecular Weight DNA isolated at pH 10.5Nature New Biology, 1972
- On the Size of the DNA in the Mammalian ChromosomeBiophysical Journal, 1970
- A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pHExperimental Cell Research, 1970
- Characterization of Macromolecules by Constant Velocity SedimentationNature, 1967