Virus-Specific Nucleic Acids in Simian Virus 40-Transformed Hamster Cell Clones Varying in Oncogenic Potential

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Abstract
The virus-specific RNA and DNA of 2 clonal lines of simian virus 40 (SV40)-transformed hamster heart cells differing in oncogenic potential were studied by nucleic acid hybridization techniques. Reconstruction experiments, with radiolabeled SV40 complementary RNA synthesized in vitro used as a probe, indicated 2.0 viral genome-equivalents of SV40-specific DNA per cell in the line with high oncogenic potential and 1.3 viral genome-equivalents per cell in the line with low oncogenic pofentic]. Cells of the low oncogenicity line appeared to synthesize less than one-fourth as much SV40-specific RNA as did cells of the high oncogenicity line, but qualitative differences could not be demonstrated.

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