Alkaline Phosphatase in Leucocytes by An Azo Dye Method
- 1 January 1959
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 34 (6) , 339-342
- https://doi.org/10.3109/10520295909114702
Abstract
Alkaline phosphatase activity was demonstrated in the cytoplasm of leucocytes of man, rat and rabbit by means of naphthol AS phosphate as the substrate and fast blue BBN as the coupling agent. The reaction was effected in Tris buffer, 0.2 M at ph 9.1, in 5-10 min at 25°C. Air-dried smears, with or without fixation in 10% neutral formalin, showed activity but those heated 1 min in water at 100°C did not.Keywords
This publication has 9 references indexed in Scilit:
- Histochemical Comparison of Naphthol AS-Phosphates for the Demonstration of PhosphatasesJNCI Journal of the National Cancer Institute, 1958
- HISTOCHEMICAL DEMONSTRATION OF β-D-GALACTOSIDASE IN THE RATJournal of Histochemistry & Cytochemistry, 1958
- Leukocytic Alkaline PhosphataseBlood, 1957
- A Histochemical Procedure for Localizing and Evaluating Leukocyte Alkaline Phosphatase Activity in Smears of Blood and MarrowBlood, 1955
- Alkaline Phosphatase Activity of Leukocytes in ShockBlood, 1955
- STUDIES ON LEUKOCYTE ALKALINE PHOSPHATASE ACTIVITY - RELATION TO STRESS AND PITUITARY-ADRENAL ACTIVITY1954
- ALKALINE PHOSPHATASE ACTIVITY IN NORMAL AND ABNORMAL HUMAN BLOOD AND BONE MARROW CELLS1946