Gas-chromatographic determination of levamisole in human plasma-normalization and reliability of the method

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Abstract
Levamisole has been used by many authors as an immunostimulant agent but the relationship between oral doses, plasma level and the therapeutic effects were not known. For such a study it was necessary to establish an accurate and reproducible method of measurement. We describe a modifi-cation of the gas chromatographic method of Wynants et al. (1975). The normalization of the extraction process leads to greater reliability and accuracy (4 ng±7.6 %). Reproducibility was established using a series of 30 determinations from standard solutions and plasmas supplemented with Levamisole. The relative standard deviation in K evaluation was varied between 0.9 and 1.8%, 3.7 and 6.1% for standard solutions and plasmas respectively. Consequently this method was adopted for this pharmacokinetic study which was carried out on healthy and cancer patients.