Fucose in 1H COSY spectra of plasma membrane fragments shed from human malignant colorectal cells

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Abstract
The methyl-methine cross peak of bound fucose has been assigned in the COSY spectrum of plasma membrane shed from human malignant colorectal cells. This cross peak (1.33-4.27 ppm), which is superimposed on the methyl-methine cross peak of threonine, was assigned following hydrolysis of the sample. Acid hydrolysis led to a 28 ± 5% reduction in the intensity of the cross peak and the apperance of the α and ß forms of fucose. Chemical analysis confirmed the release of free fucose. Lactate anion. which was not perturbed by the hydrolysis, was used as an internal standard. © 1991 Academic Press, Inc.