Characterization of two monoclonal antibodies directed against the 67 kDa high affinity laminin receptor and application for the study of breast carcinoma progression
- 1 November 1992
- journal article
- research article
- Published by Springer Nature in Clinical & Experimental Metastasis
- Vol. 10 (6) , 379-386
- https://doi.org/10.1007/bf00133466
Abstract
Two monoclonal antibodies (mAbs), designated MLuC5 and MLuC6, were produced against a human small cell lung carcinoma cell line. They were found to exhibit a superimposable reactivity on different cell lines and on platelets. Moreover, they both immunoprecipitated a 67 kDa molecule from the membrane of the reference target cells. Immunodepletion and cross-inhibition tests indicated that the two mAbs recognize two epitopes closely localized on the same molecule. The MLuC5 mAb was further characterized for its reactivity on platelets. Immunoprecipitation and ELISA assays demonstrate that this mAb recognizes the 67 kDa high afinity laminin receptor. MLuC5 reactivity was evaluated by immunohistochemistry on a variety of normal and tumor tissues, in particular breast specimens including normal epithelium, dysplastic lesions, in situ carcinomas, invasive primary carcinomas and distant metastases. The laminin receptor was found to be strongly expressed in 50% of the infiltrating carcinomas, whereas in situ carcinomas and benign lesions, as well as the normal mammary epithelium, were only weakly and focally positive. In metastatic lesions MLuC5 reactivity was only found in 11% of the samples tested, independently of the site of origin of the lesion.Keywords
This publication has 35 references indexed in Scilit:
- Characterization of a monoclonal antibody directed against the epidermal growth factor receptor binding siteCancer Immunology, Immunotherapy, 1991
- Receptor functions for the integrin VLA-3: fibronectin, collagen, and laminin binding are differentially influenced by Arg-Gly-Asp peptide and by divalent cations.The Journal of cell biology, 1991
- Modulation of Laminin Receptor Expression by Estrogen and Progestins in Human Breast Cancer Cell LinesJNCI Journal of the National Cancer Institute, 1989
- Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor bindingCell, 1987
- Laminin molecular domains which alter metastasis in a murine model.Journal of Clinical Investigation, 1984
- Laminin — a multifunctional protein of basement membranesTrends in Biochemical Sciences, 1983
- Isolation of a tumor cell laminin receptorBiochemical and Biophysical Research Communications, 1983
- Shapes, domain organizations and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrixJournal of Molecular Biology, 1981
- Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-SepharoseImmunochemistry, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970