Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
- 1 January 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (1) , 76-80
- https://doi.org/10.1073/pnas.79.1.76
Abstract
The sizes and relative map positions of RNA molecules encoded by the plasmid DNA of several octopine-type tobacco crown gall tumors [Agrobacterium tumefaciens] were determined by blot hybridization. At least 6 discrete polyadenylylated RNA (sizes, 0.73-1.75 kilobases) were detected in octopine-producing tumors (lines A6S/2, E9, and 15955/01). In 1 tumor (line 15955/1) in which octopine could not be detected, one of the RNA species was missing and another was diminished in abundance. Evidence is presented suggesting that the transcripts are encoded entirely by the plasmid DNA and do not originate from the surrounding plant DNA sequences.This publication has 19 references indexed in Scilit:
- The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiaeNucleic Acids Research, 1980
- Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciensProceedings of the National Academy of Sciences, 1980
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- Proteins encoded by Agrobacterium tumefaciens Ti plasmid DNA (T-DNA) in crown gall tumorsProceedings of the National Academy of Sciences, 1980
- Purification and Characterization of the Crown Gall-specific Enzyme, Octopine SynthasePlant Physiology, 1980
- The functional organization of the nopaline A. tumefaciens plasmid pTiC58Plasmid, 1980
- Integration and organization of Ti plasmid sequences in crown gall tumorsCell, 1980
- Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNANucleic Acids Research, 1980
- Transcription of Ti plasmid-derived sequences in three octopine-type crown gall tumor lines.Proceedings of the National Academy of Sciences, 1979
- Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.Proceedings of the National Academy of Sciences, 1977