Blast Transformation of Rabbit B-Derived Lymphocytes by a Mitogen Extracted from Nocardia
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 114 (1_Part_2) , 348-353
- https://doi.org/10.4049/jimmunol.114.1_part_2.348
Abstract
Nocardia water soluble mitogen (NWSM) is known to stimulate mouse and rabbit lymphoid cells and to act selectively on murine B-derived lymphocytes. In this paper, evidence is presented that NWSM is also a mitogen for rabbit bursal equivalent cells and does not bring about blast transformation of thymus-derived rabbit cells. Pretreatment of rabbit spleen lymphocytes with antibody directed against rabbit thymus lymphocyte antigen (anti-RTLA serum) and with complement did not affect the strong increase of thymidine incorporation which follows stimulation with NWSM. The mitogen-induced polyclonal activation of antibody-forming cells and resulted in the presence of 30% of cells with the ultrastructural characteristics of plasmocytes. These observations led to the conclusion that NWSM is a mitogen for a B-derived lymphocyte of the rabbit.This publication has 0 references indexed in Scilit: