Rapid and dense culture of Acinetobacter calcoaceticus on palm oil.

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Abstract
A soil microorganism, identified as Acinetobacter calcoaceticus KB-2, was cultivated on palm oil as a carbon source for cell production. This organism grew with a specific growth rate of 1.10h-1. The pH optimum for growth was between 6.5 and 7.0, and the temperature optimum was 39°C. Compared with other strains on water-insoluble substrates such as hydrocarbons and natural oils and fats so far reported, the cultivation time for this strain was short and the cell mass productivity was relatively high. More than 90% of the palm oil was assimilated by this strain, and the overall cell yield was 1.02 (g of cells/g of palm oil) after 8 hr cultivation with the concentration of 3% palm oil.

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