In Vivo1H‐NMR microimaging with respiratory triggering for monitoring adoptive immunotherapy of metastatic mouse lymphoma

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Abstract
The metastatic ESb‐MP murine lymphoma in DBN2 mice has been used as a model for investigating metastatic disease and its cure by adoptive immunotherapy (ADI) as monitored by in vivo multislice spin‐echo 1H NMR microimaging at 7 T. Isoflurane inhalation anesthesia facilitated long measurement sessions, and respiratory gating with a fiber‐optic sensor greatly reduced motional artifacts. With T2 weighting (TR = 2 s, TE = 30 ms) mean signal‐to‐noise ratios of 30 and 15 for kidney and liver, respectively, were achieved in 20 min (100‐μm pixels, 1‐mm slices, 25‐mm field of view). Without the use of contrast agents, metastases with diameters > 0.3 mm in the imaged plane could be detected as hyperintense lesions in kidney (contrast ratio ca. 1.4) and liver (contrast ratio ca. 2) with a confidence level of > 98%. For the first time the complete eradication of late‐stage macroscopic metastases by ADI could be demonstrated noninvasively by MRI.