Factors Affecting the Reactivity of Human Lymphocytes in Vitro

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Abstract
Summary and Conclusions: Quantitative studies of immunologically stimulated blood leukocytes in vitro show that DNA synthesis is affected by variations in cell number, duration of culture and area of the surface on which the cells are cultured. Of particular interest is the demonstration in both mixed leukocyte and antigen-stimulated cultures that as cell proximity increases the synthesis of DNA increases proportionally. It is suggested that this increase in DNA synthesis is the result of recruitment of otherwise nonreactive lymphocytes through the previously demonstrated mechanism of uropodapsis.

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