Regulation of CAPRICE Transcription by MYB Proteins for Root Epidermis Differentiation in Arabidopsis
Open Access
- 1 June 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 46 (6) , 817-826
- https://doi.org/10.1093/pcp/pci096
Abstract
Epidermal cell differentiation in Arabidopsis root is studied as a model system for understanding cell fate specification. Two types of MYB-related transcription factors are involved in this cell differentiation. One of these, CAPRICE (CPC), encoding an R3-type MYB protein, is a positive regulator of hair cell differentiation and is preferentially transcribed in hairless cells. We analyzed the regulatory mechanism of CPC transcription. Deletion analyses of the CPC promoter revealed that hairless cell-specific transcription of the CPC gene required a 69 bp sequence, and a tandem repeat of this region was sufficient for its expression in epidermis. This region includes two MYB-binding sites, and the epidermis-specific transcription of CPC was abolished when base substitutions were introduced in these sites. We showed by gel mobility shift experiments and by yeast one-hybrid assay that WEREWOLF (WER), which is an R2R3-type MYB protein, directly binds to this region. We showed that WER also binds to the GL2 promoter region, indicating that WER directly regulates CPC and GL2 transcription by binding to their promoter regions.Keywords
This publication has 32 references indexed in Scilit:
- A network of redundant bHLH proteins functions in all TTG1-dependent pathways ofArabidopsisDevelopment, 2003
- Cell Pattern in the Arabidopsis Root Epidermis Determined by Lateral Inhibition with FeedbackPlant Cell, 2002
- The TRANSPARENT TESTA GLABRA1 Locus, Which Regulates Trichome Differentiation and Anthocyanin Biosynthesis in Arabidopsis, Encodes a WD40 Repeat ProteinPlant Cell, 1999
- Evidence for direct activation of an anthocyanin promoter by the maize C1 protein and comparison of DNA binding by related Myb domain proteins.Plant Cell, 1997
- Differences between Plant and Animal Myb Domains Are Fundamental for DNA Binding Activity, and Chimeric Myb Domains Have Novel DNA Binding SpecificitiesPublished by Elsevier ,1997
- The Arabidopsis Athb‐10 (GLABRA2) is an HD‐Zip protein required for regulation of root hair developmentThe Plant Journal, 1996
- Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helicesCell, 1994
- The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis.Genes & Development, 1994
- The myb-homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subsetCell, 1994
- Reversible Root Tip Rotation in Arabidopsis Seedlings Induced by Obstacle-Touching StimulusScience, 1990