Enhancing effects of CO2 on chloroplast regeneration in glucose-bleached cells of Chlorella protothecoides I. Role of non-photosynthetic CO2-fixation in chloroplast regeneration1

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Abstract
Carbon dioxide enhanced chloroplast regeneration in glucose-bleached cells of Chlorella protothecoides in the presence of CMU in the light. Both the formation of chlorophyll and the syntheses of RNA and protein were considerably enhanced. The CO2 metabolism of algal cells during greening was investigated using 14C-bicarbonate as the tracer. Radiocarbon was largely incorporated into purine and pyrimidine bases in nucleic acid and the arginine in protein, specifically at the crabon atoms derived from carbamylphosphate.