THE DETERMINATION OF LIVER VITAMIN B12 AND FOLATE ACTIVITY ON A SINGLE EXTRACT

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Abstract
When assaying vitamin B12 (with Euglena gracilis) and folate (with Lactobacillus easel) activity of material obtained by needle biopsy of human liver, it is important because of the small size of the sample to choose an extraction method suitable for both estimations. The release of vitamin B12 from the bound form depends on heat rather than enzyme extraction, whereas the release of folate active material requires preliminary treatment with enzymes such as papain or chicken pancreas. Both materials were tested at various pH levels and both gave comparable results. However, the papain contained no vitamin B12 activity, less folate activity than chicken pancreas, and the added advantage of requiring less heat treatment.

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